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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
01/09/2020 |
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Data da última atualização: |
19/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. |
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Afiliação: |
THUANNE PIRES RIBEIRO, UNB; MARCOS FERNANDO BASSO; MAYARA HOLANDA DE CARVALHO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; DAGNA MARIA LAURINDO DA SILVA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OSMUNDO BRILANTE DE OLIVEIRA-NETO; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; BRUNA MENDES DINIZ TRIPODE, CNPA; TATIANNE PIZA FERRARI ABREU-JARDIM; JOSE EDNILSON MIRANDA, CNPA; MARCIO ALVES-FERREIRA, UFRJ; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 3, p. 27-41, 2020. |
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DOI: |
https://doi.org/10.1016/j.biori.2019.12.003 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW. MenosThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events ... Mostrar Tudo |
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Palavras-Chave: |
Bacillus; Bicudo do algodão; Entomotoxin; Herança estável; Insect pest; Management; Praga de inseto; Resistance; Resistência gestão; Stable inheritance; Thuringiensis. |
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Thesagro: |
Algodão; Inseto; Praga; Praga de Planta. |
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Thesaurus Nal: |
Cotton; Insect pests; Resistance management. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216823/1/Stability-and-tissue-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215669/1/1-s2.0-S2452072119301352-main.pdf
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Marc: |
LEADER 03317naa a2200529 a 4500
001 2125667
005 2020-11-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biori.2019.12.003$2DOI
100 1 $aRIBEIRO, T. P.
245 $aStability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil.$h[electronic resource]
260 $c2020
520 $aThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW.
650 $aCotton
650 $aInsect pests
650 $aResistance management
650 $aAlgodão
650 $aInseto
650 $aPraga
650 $aPraga de Planta
653 $aBacillus
653 $aBicudo do algodão
653 $aEntomotoxin
653 $aHerança estável
653 $aInsect pest
653 $aManagement
653 $aPraga de inseto
653 $aResistance
653 $aResistência gestão
653 $aStable inheritance
653 $aThuringiensis
700 1 $aBASSO, M. F.
700 1 $aCARVALHO, M. H. de
700 1 $aMACEDO, L. L. P. de
700 1 $aSILVA, D. M. L. da
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aOLIVEIRA-NETO, O. B. de
700 1 $aCAMPOS-PINTO, E. R. de
700 1 $aLUCENA, W. A.
700 1 $aSILVA, M. C. M. da
700 1 $aTRIPODE, B. M. D.
700 1 $aABREU-JARDIM, T. P. F.
700 1 $aMIRANDA, J. E.
700 1 $aALVES-FERREIRA, M.
700 1 $aMORGANTE, C. V.
700 1 $aGROSSI-DE-SA, M. F.
773 $tBiotechnology Research and Innovation$gv. 3, p. 27-41, 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 10 | |
| 1. |  | BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; BUSANELLO, C.; PINTO, C. E. M.; FREITAS, E. de O.; RIBEIRO, T. P.; ENGLER, J. de A.; OLIVEIRA, A. C. de; MORGANTE, C. V.; ALVES-FERREIRA, M.; GROSSI-DE-SA, M. F. Insights obtained using different modules of the cotton uceA1.7 promoter. Planta, v. 251, n. 2, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 2. | | ROCA PAIXÃO, J. F.; GILLET, F. X.; RIBEIRO, T. P.; BOURNAUD, C.; LOURENCO-TESSUTTI, I. T.; NORIEGA, D. D.; MELO, B. P. de; ALMEIDA-ENGLER, J. de; GROSSI-DE-SA, M. F. Improved drought stress tolerance in Arabidopsis by CRISPR/ dCas9 fusion with a Histone AcetylTransferase. Scientific Reports, v. 9, n. 1, p. 1-9, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; MENDES, R. A. G.; PINTO, C. E. M.; BOURNAUD, C.; GILLET, F.-X.; TOGAWA, R. C.; MACEDO, L. L. P. de; ENGLER, J. d A.; GROSSI-DE-SA, M. F. MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita. Scientific Reports, v. 10, n. 1, p. 1-13, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. Frontiers in Plant Science, v. 11, article 1228, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 5. | | MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; PAIXÃO, J. F. R.; NORIEGA, D. D.; SILVA, M. C. M.; ALMEIDA-ENGLER, J. de; FONTES, E. P. B.; GROSSI-DE-SA, M. F. Transcriptional modulation of AREB-1 by cRiSpRa improves plant physiological performance under severe water deficit. Scientific Reports, v. 10, 16231, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | FREITA, E. O.; MELO, B. P.; LOURENCO-TESSUTTI, I. T.; ARRAES, F. B. M.; AMORIM, R. M.; LISEI-DE-SÁ, M. E.; COSTA, J. A.; LEITE, A. G. B.; FAHEEM, M.; FERREIRA, M. A.; MORGANTE, C. V.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application. BMC Biotechnology, v. 19, article 79, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 7. | | FIRMINO, A. A. P.; PINHEIRO, D. H.; MOREIRA-PINTO, C. E.; ANTONINO, J. D.; MACEDO, L. L. P.; MARTINS-DE-SA, D.; ARRAES, F. B. M.; COELHO, R. R.; FONSECA, F. C. de A.; SILVA, M. C. M.; ENGLER, J. de A.; SILVA, M. S.; LOURENÇO-TESSUTTI, I. T.; TERRA, W. R.; GROSSI-DE-SA, M. F. RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis). Frontiers in Physiology, v. 11, article 591569, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO-TESSUTTI, I. T.; SILVA, M. S.; LISEI-DE-SÁ, M. E.; LUCENA, W. A.; MACEDO, L. L. P. de; LIMA, J. N.; AMORIM, R. M. S.; ARTICO, S.; ALVES-FERREIRA, M.; SILVA, M. C. M.; GROSSI-DE-SA, M. F. Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil. Plant Biotechnology Journal, v. 15, p. 997-1009, 2017. (Open Access).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B. M.; LUCENA, W. A.; LOURENCO TESSUTTI, I. T.; BARBOSA, A. A. de D.; SILVA, M. C. M. da; GROSSI DE SA, M. F. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science, v. 7, article 165 , 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnology Research and Innovation, v. 3, p. 27-41, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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